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KMID : 0380020180330020110
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 2 p.110 ~ p.117
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Optimization of Culture Conditions for the Production of Mycelial Biomass and Polysaccharide with Inonotus obliquus in Liquid Culture
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Song In-Kyu
Choi Byung-Hyun
Hong Eock-Kee
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Abstract
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This study was carried out to investigate the optimum liquid culture conditions for the mycelium and the polysaccharide production on liquid culture of Inonotus obliquus. In flask culture, the production of maximum mycelium and polysaccharide were 9.23 g/L and 1.85 g/L, respectively. In bioreactor, with the culture conditions of agitation rate 200 rpm and aeration rate 1 vvm, the production of mycelium and polysaccharide were 14.17 g/L and 2.81 g/L at the 8th day of cultivation, respectively. And the cultivation was operated with 300 rpm and 1 vvm up to the 4th day of cultivation, since then, 200 rpm and 1 vvm, resulting in that the production of mycelium was 15.3 g/L at the 9th day and the polysaccharide production was 2.93 g/L at the 6th day of the cultivation. Furthermore, with the same agitation and aeration rates as the above during the cultivation, the addition of glucose being 20 g/L of the final concentration was intermittently accomplished in order to increase the mycelium and the polysaccharide production. As a result, the addition of glucose at the 3rd day of cultivation resulted in that the maximum mycelium and the polysaccharide production were 17.1 g/L and 3.4 g/L at the 7th day of cultivation, respectively, indicating that the cultivation period was 4 days shorter than the cultivation of 11 days.
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KEYWORD
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Inonotus obliquus, mycelia, polysaccharide, liquid culture, bioreactor
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